Review



affigel blue column  (Millipore)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Millipore affigel blue column
    (A) Denaturing gel analysis of the purified PAP fractions from <t>Affigel</t> Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.
    Affigel Blue Column, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affigel blue column/product/Millipore
    Average 90 stars, based on 1 article reviews
    affigel blue column - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Characterization of a Soluble Phosphatidic Acid Phosphatase in Bitter Melon ( Momordica charantia )"

    Article Title: Characterization of a Soluble Phosphatidic Acid Phosphatase in Bitter Melon ( Momordica charantia )

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0106403

    (A) Denaturing gel analysis of the purified PAP fractions from Affigel Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.
    Figure Legend Snippet: (A) Denaturing gel analysis of the purified PAP fractions from Affigel Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.

    Techniques Used: Purification, SDS Page, Staining, Activity Assay, Phosphatase Assay, Positive Control, Marker



    Similar Products

    deae affigel blue column  (Bio-Rad)
    93
    Bio-Rad deae affigel blue column
    Deae Affigel Blue Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deae affigel blue column/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    deae affigel blue column - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    affigel blue column  (Bio-Rad)
    96
    Bio-Rad affigel blue column
    Affigel Blue Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affigel blue column/product/Bio-Rad
    Average 96 stars, based on 1 article reviews
    affigel blue column - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    affigel blue column  (Millipore)
    90
    Millipore affigel blue column
    (A) Denaturing gel analysis of the purified PAP fractions from <t>Affigel</t> Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.
    Affigel Blue Column, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affigel blue column/product/Millipore
    Average 90 stars, based on 1 article reviews
    affigel blue column - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    deae-affigel blue column  (Bio-Rad)
    90
    Bio-Rad deae-affigel blue column
    (A) Denaturing gel analysis of the purified PAP fractions from <t>Affigel</t> Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.
    Deae Affigel Blue Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deae-affigel blue column/product/Bio-Rad
    Average 90 stars, based on 1 article reviews
    deae-affigel blue column - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Denaturing gel analysis of the purified PAP fractions from Affigel Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.

    Journal: PLoS ONE

    Article Title: Characterization of a Soluble Phosphatidic Acid Phosphatase in Bitter Melon ( Momordica charantia )

    doi: 10.1371/journal.pone.0106403

    Figure Lengend Snippet: (A) Denaturing gel analysis of the purified PAP fractions from Affigel Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.

    Article Snippet: Enzyme fraction from Affigel Blue column was concentrated in a Centricon concentrator (Amicon, centrifugal filter devices, Millipore Corporation) and during the course the imidazole buffer was exchanged to protease digestion buffer (50 mM ammonium carbonate, pH 8.0).

    Techniques: Purification, SDS Page, Staining, Activity Assay, Phosphatase Assay, Positive Control, Marker